Li Qingwang

Qing-Wang Li, Ph.D. Professor

College of Animal Science and Technology
Animal Reproduction and Cell Engineering Laboratory

Mailing Address:
College of Animal Science & Technology
Northwest A&F University
22 St.XiNong, YangLing, Shaan'xi, P.R. of China (712100)

Telephone: 029-8703278513709129117


1982 Bachelor of Agriculture in Animal Husbandry, Northwest Agricultural University

1985 Master of Agriculture in Animal Reproduction, Northwest Agricultural University

1993 Doctor of Agriculture in Animal obstetrics, Northwest Agricultural University

1993-1995 Study Abroad, Danish National Animal Husbandry Institute and The Royal Veterinary and Agricultural University

1995-1996 Post Doctoral Research in Transgenic Animal, Southern Illinois University, U.S.A.

Research Interests:

1. In the field of transgenic animals and protein drugs of genetic engineering

(1) The new animal transgenic technology was established and the transgenic mice with green fluorescent protein was born.

(2) Transgenic protein drugs have been produced using animal mammary gland, mainly including Human Lactoferrin Protein, Human Nerve Growth Factor, Antithrombin III and so on. The technology can be used for mass production of protein drugs in the factory.

2. In the field of animal reproductive physiology and control technology

(1) Establish a stable and efficient embryo transfer and IVF technology system in cattle and sheep, the blastocyst rate was 38% and success rate of embryo transfer was up to 50%.

(2) In the field of germ cell freezing, the survival rate of frozen-thawed sperm (pig, cattle, sheep) reached 0.50 to 0.60, survival rate of pig oocytes was 23%, and two new type natural antifreezes were found.

(3) Establish isolation and culture methods of spermatogonial cell in vitro, and directed differentiation in vitro is being studied.

3. The research of anti-cancer and hypoglycemic drugs in cancer and diabetes associated with animal reproduction and infertility

(1) Two kinds of natural anti-cancer drugs were found, they had obvious anti-cancer effect.

(2) Four kinds of good natural hypoglycemic drugs were found.

(3) The research of genetic engineering drugs in anti-cancer and anti-diabetes has also made significant progress.


Animal Reproduction; Principles of Animal Reproduction; Animal Reproduction Technology; Animal Reproduction Immunology; Progress of Modern Animal Genetic Breeding; Modern Animal Biotechnology; Animal Cell Engineering


1. Dairy Key technology integration and industrialization demonstration. Science and Technology Progress Award by Shaanxi Province. The Third one. 2008

2. Guidance of doctoral dissertation: Study on Cryopreservation of Boar Semen. Outstanding Paper Award by Shaanxi Province. 2008

Outreach and Extension:


1. Research on production of genetic engineering drugs by transgenic technology of Qinhuangdao Technology Investment Corporation

2. Research and development on ecological dairy industry and sex control technology of dairy cattle in northwest rural area (No.2006BAD04A11) of National Key Technology R&D Program

3. The fast and efficient breeding technology integration of high-yield dairy cattle and industrialization demonstration (2007-27) of Agricultural Science and Technology Project

4. Research on transgenic animal with disease resistance (2009ZX08007-008B) of Major Technology Project of Ministry of Agriculture

5. Development and application of livestock sperm separation with rapid and lower damage (No.2008AA101004) of National High-Tech Research and Development Project

6. Research on dairy cattle efficient breeding and industrialization demonstration (No.2009ZDKG-17) of Technology Innovation Project (“13115”) by Science and Technology Department of Shaanxi Province


SCI included:

  1. Long L, Li QW. The effect of alkaloid from Oxytropis ochrocephala on growth inhibition and expression of PCNA and p53 in mice bearing H22 hepatocellular carcinoma. Yakugaku Zasshi, 2005, 125(8): 665-670.
  2. Zhao R, Li QW, Xiao B. Effect of Lycium barbarum Polysaccharide on the Improvement of Insulin Resistance in NIDDM Rats. Yakugaku Zasshi, 2005, 125(12): 981-988.
  3. Hu JH, Li QW, Li G, Chen XY, Yang H, Zhang SS, Wang LQ. The cryoprotective effect on frozen-thawed boar semen of egg yolk low density lipoproteins. Asian-Australasian Journal of Animal Sciences.2006, 19(4):486-494. (IF=0.854)
  4. Han ZS, Li QW, Zhang ZY, Xiao B, Gao DW, Wu SY, Li J, Zhao HW, Jiang ZL, Hu JH. High-level expression of human lactoferrin in the milk of goats by using replication-defective adenoviral vectors. Protein Expr Purif. 2007 May; 53(1):225-231. (IF=1.867)
  5. Jiang ZL, Li QW, Li WY, Hu JH, Zhao HW, Zhang SS. Effect of low density lipoprotein on DNA integrity of freezing-thawing boar sperm by neutral comet assay. Anim Reprod Sci. 2007 Jun; 99(3-4):401-407. (IF=2.186)
  6. Jiang ZL, Li QW, Hu JH, Li WY, Zhao HW, Zhang SS. Improvement of the quality of boar cryopreservation semen by supplementing with low density lipoprotein in diluents. Cryobiology. 2007 Jun; 54(3):301-304. (IF=2.162)
  7. Chen XY, Li QW, Zhang SS, Han ZS, Zhao R, Wu SY, Huang J. Effects of ovarian cortex cell co-culture during in vitro maturation on porcine oocytes maturation, fertilization and embryo development. Anim Reprod Sci. 2007 Jun; 99(3-4): 306-316. (IF=2.186)
  8. Zhao R, Li Q, Long L, Li J, Yang R, Gao D. Anti-diabetic activity of flavone from Ipomoea Batatas leaf in non-insulin dependent diabetic rats. International journal of food science and technology.2007, 42: 80-85. (IF=0.832)
  9. Li K, Li Q, Li J, Zhang T, Han Z, Gao D, Zheng F. Antitumor activity of the procyanidins from Pinus koraiensis bark on mice bearing U14 cervical cancer. Yakugaku Zasshi. 2007 Jul; 127(7):1145-1151. (IF=0.225)
  10. Li K, Li Q, Li J, Gao D, Zhang T, Han Z, Zheng F. Effect of procyanidins from Pinus koraiensis bark on growth inhibition and expression of PCNA and TNF-α in mice with U14 cervical cancer. Therapy. 2007, 4(5): 685-690.
  11. Li J, Li Q, Feng T, Zhang T, Li K, Zhao R, Han Z, Gao D. Antitumor activity of crude polysaccharides isolated from Solanum nigrum Linne on U14 cervical carcinoma bearing mice. Phytother Res. 2007 Sep; 21(9):832-840. (IF=1.144)
  12. Gao D, Li Q, Liu Z, Li Y, Liu Z, Fan Y, Li K, Han Z, Li J. Hypoglycemic effects and mechanisms of action of Cortex Lycii Radicis on alloxan-induced diabetic mice. Yakugaku Zasshi. 2007 Oct; 127(10): 1715-1721. (IF=0.225)
  13. Gao D, Li Q, Li Y, Liu Z, Liu Z, Fan Y, Han Z, Li J, Li K. Antidiabetic potential of oleanolic acid from Ligustrum lucidum Ait. Can J Physiol Pharmacol. 2007, 85: 1-8. (IF=1.587)
  14. Gao D, Li Q, Liu Z,Li Y,Liu Z, Fan Y, Han Z ,Li J. Effects of Lycium barbarum L. root bark extract on alloxan-induced diabetic mice. Therapy. 2007, 4(5): 547-553. (IF=0.225)
  15. Han ZS, Li QW, Zhang ZY, Yu YS, Xiao B, Wu SY, Jiang ZL, Zhao HW, Zhao R, Li J. Adenoviral vector mediates high expression levels of human lactoferrin in the milk of rabbits. J Microbiol Biotechnol. 2008 Jan; 18(1): 153-159. (IF=2.062)
  16. Xiao B, Li QW, Feng B, Han ZS, Gao da W, Li J, Li K, Zhao R, Jiang ZL, Hu JH, Zhi XB. High-level expression of recombinant human nerve growth factor beta in milk of nontransgenic rabbits. J Biosci Bioeng. 2008 Apr; 105(4):327-334. (IF=1.782)
  17. Xiao B, Li QW, Feng B, Han ZS, Gao DW, Zhao R, Li J, Li K, Zhi XB, Yang H, Liu ZB. Expression of Recombinant Human Nerve Growth Factor Beta in Milk of Goats by Recombinant Replication-Defective Adenovirus. Appl Biochem Biotechnol. 2008 Jun; 157: 357-366. (IF=1.643)
  18. Zhang T, Li QW, Li K, Li Y, Li J, Wang G, Zhou S. Antitumor effects of saponin extract from Patrinia villosa (Thunb.) Juss on mice bearing U14 cervical cancer. Phytother Res. 2008 May; 22(5): 640-645. (IF=1.43)
  19. Gao D, Li N , Li Q, Li J, Han Z , Fan Y, Liu Z. Study of the extraction, purification and antidiabetic potential of ursolic acid from Cornus officinalis Sieb. et Zucc. Therapy. 2008, 5(5): 697-705.
  20. Huang J, Li Q, Zhao R, Li W, Han Z, Chen X, Xiao B, Wu S, Jiang Z, Hu J, Liu L. Effect of sugars on maturation rate of vitrified-thawed immature porcine oocytes. Anim Reprod Sci. 2008 Jun; 106(1-2):25-35. (IF=1.739)
  21. Li J, Li QW, Gao DW, Han ZS, Li K. Antitumor effects of total alkaloids isolated from Solanum nigrum in vitro and in vivo. Pharmazie. 2008 Jul; 63(7):534-538. (IF=0.101)
  22. Li J, Li QW, Feng T, Li K. Aqueous extract of Solanum nigrum inhibit growth of cervical carcinoma (U14) via modulating immune response of tumor bearing mice and inducing apoptosis of tumor cells. Fitoterapia. 2008 Dec; 79(7-8): 548-556. (IF=1.106)
  23. Li K, Li QW, Han ZS, Li J, Gao D, Liu Z, Zheng F. Alkaloid from Angelicae dahuricae inhibits HeLa cell growth by inducing apoptosis and increasing caspase-3 activity. Lab Med. 2008, 39: 540-546. (IF=0.197)
  24. Hu JH, Li QW, Jiang ZL, Li WY. Effects of different extenders on DNA integrity of boar spermatozoa following freezing-thawing. Cryobiology. 2008 Dec; 57(3): 257-262. (IF=1.936)
  25. Hu JH, Li QW, Li G, Jiang ZL, Bu SH, Yang H, Wang LQ. The cryoprotective effect of trehalose supplementation on boar spermatozoa quality. Anim Reprod Sci. 2009 May; 112(1-2): 107-118. (IF=1.739)
  26. Hu JH, Li QW, Li G, Jiang ZL, Bu SH, Yang H, Wang LQ. The cryoprotective effect of trehalose supplementation on boar spermatozoa quality. Reproduction in Domestic Animals. 2009, 44: 571-575.
  27. Hu JH, Li QW, Zhang T, Jiang ZL. Effect of Gynostemma Pentaphyllum Polysaccharide on Boar Spermatozoa Quality Following Freezing-thawing. Cryobiology. 2009 May; 59: 244-249. (IF=1.936)
  28. Hu JH, Li QW, Chen YL, Jiang ZL, Jia YH, Wang LQ, Ou BB. Effects of addition of vitamin B12 to the extender on post-thaw motility, acrosome morphology, and plasma membrane integrity in bull semen. Turk. J. Vet. Anim. Sci, 2009, 33(5): 379-384.
  29. Han ZS, Wu SY, Li QW, Li J, Gao DW, Li K, Liu ZW, Zhao HW. Efficient Human Growth Hormone gene expression in the Milk of non-transgenic Goats, Folia Biol. (Praha). 2009, 55: 17-22. (IF=1.14)
  30. Gao DW, Li QW, Li Y, Liu ZH, Fan YS, Liu ZW, Zhao HW, Li J, Han ZS. Antidiabetic and antioxidant effects of oleanolic acid from Ligustrum lucidum Ait in alloxan-induced diabetic rats. Phytother Res. 2009 Mar; 23: 1257-1262. (IF=1.43)
  31. Li J, Li QW, Gao DW, Han ZS, Lu WZ. Antitumor and immunomodulating effects of polysaccharides isolated from Solanum nigrum Linne, Phytother. Res. 2009, 23: 1524–1530. (IF=1.43)
  32. Lu WZ, Li QW, Li J, Liu FZ, Yang XL. Polysaccharide from Patrinia heterophylla Bunge Inhibits HeLa Cell Proliferation Through Induction of Apoptosis and Cell Cycle Arrest. Lab Med. 2009, 40(3): 161-166. (IF=0.21)
  33. Lu WZ, Yang YL, Li QW, Liu FZ. Crude Flavonoids from Carya cathayensis Sargent inhibited HeLa cells proliferation through induction of apoptosis and cell cycle arrest. Latin American Journal of Pharmacy. 2009, 28(4): 568-573.
  34. Lu WZ, Geng GX, Li QW, Li J, Liu FZ, Han ZS, Gao DW, Yan X, Yang XL. Anti-tumor Activity of Polysaccharides isolated from Patrinia scabra Bunge on U14 Cervical Carcinoma Bearing Mice. The American Journal of Chinese Medicine. 2009, 37(5): 933-944. (IF=1.058)
  35. Yang H, Li QW, Han ZS, Hu JH, Li WY, Liu ZB. Recombinant human antithrombin expressed in the milk of non-transgenic goats exhibits high efficiency on rat DIC model. J Thromb Thrombolysis. 2009 Nov; 28(4): 449–457(IF=2.266)
  36. Li FL, Li QW, Gao DW, Peng Y, Feng CN. Preparation and antidiabetic activity of polysaccharide from Portulaca oleracea L. African Journal of Biotechnology. 2009 Feb; 8 (4): 569-573. (IF=0.456)
  37. Li FL, Li QW, Gao DW, Peng Y. The Optimal Extraction Parameters and Anti-Diabetic Activity of Flavonoids from Ipomoea Batatas Leaf .Afr. J. Trad. 2009, 6(2): 195-202.
  38. Zhao HW, Li QW, Ning GZ, Han ZS, Jiang ZL, Duan YF. Rhodiola sacra aqueous extract (RSAE) improves biochemical and sperm characteristics in cryopreserved boar semen. Theriogenology. 2009 Mar; 71: 849-857. (IF=1.911)
  39. Zhao R, Li QW, Li J, Zhang T. Protective effect of Lycium barbarum polysaccharide 4 on kidneys in streptozotocin-induced diabetic rats. Canadian Journal of Physiology and Pharmacology. 2009 Sep; 87: 711-719. (IF=1.763)
  40. Zhang SS, Hu JH, Li QW, Jiang ZL, Zhang XY. The cryoprotective effects of soybean lecithin on boar spermatozoa quality. African Journal of Biotechnology. 2009 Nov; 8(22): 6476-6480. (IF=0.456)
  41. Hu JH, Li QW, Zan LS, Jiang ZL, An JH, Wang LQ, Jia YH. The cryoprotective effect of low-density lipoproteins in extenders on bull spermatozoa following freezing–thawing. Animal Reproduction Science. 2010, 117: 11-17.
  42. Hu JH, Zhang SS, Li QW, Jiang ZL, Zhang DF. Effects of Laminaria Japonica Polysaccharide (LJP) on Boar Spermatozoa Quality Following Freezing-thawing. Animal Reproduction Science. doi:10.1016/j.anireprosci.2010.03.004.
  43. Li J, Li QW, Peng Y, Zhao R, Han ZS, Gao DW. Protective effects of fraction 1a of polysaccharides isolated from Solanum nigrum Linne on thymus in tumor-bearing mice. Journal of Ethnopharmacology. 2010, 129: 350-356.

Publishing books:

  1. Animal Cell Engineering and Practice, Beijing: Chemical Industry Press, 2005
  2. Livestock Breeding and Improvement, Higher Education Press, 2009.8
  3. Animal Reproduction, China Agricultural Press, 2009.6
  4. Animal Reproduction Technology, China Agricultural PressBeing Typeset, 2010

Patents: (National Invention Patent)

1. A kind of Chinese herbal medicine diluent for bull frozen semen (Patent No. ZL 200510043091.8, 2006)

2. A method of producing transgenic protein drug by the expression of mammary glands using adenovirus vector (Application No. 200610081469.8, 2006)

3. The use of Salidroside polysaccharide as new antifreeze and the preparation method (Application No. 200610104978.8, 2006)

4. The use of Chinese herbal medicine Ligustrum lucidum extract and the preparation method (Application No. 200610102157.0, 2006)

5. The use of Rhodiola extract and the preparation method (Application No. 200610102156.6, 2006)

6. A method to produce active protein in vitro (Application No. 200710199227.3, 2007)

7. A cryoprotectant used on cryopreservation of biological cell (Application No. 200710019124.4, 2007)

8. The use of Sweet potato leaves as oral hypoglycemic and its preparation method (Application No. 200710185235.2, 2007)

9. The use of Chinese wolfberry for the treatment of diabetic nephropathy and its preparation method (Application No. 200710185238.6, 2007)

10. A kind of diluent for bull frozen semen and its preparation method (Application No. 200810017382.3, 2008)

11. A kind of recombinant adenovirus carrying human lactoferrin and its preparation method and application (Application No. 200910021705.0, 2009)

12. A method of producing transgenic animals by using recombinant adenovirus vector (Application No. 200910021856.6, 2009)

13. The antifreeze for boar semen cryopreservation and its preparation method and application (Application No. 201010144435.5, 2010)