English

Chuanying Pan

Chuanying Pan, Professor

College of Animal Science and Technology,

Northwest A&F University (NWSUAF),

No. 22 Xinong Road, Yangling, Shaanxi Province, 712100, China

Telephone: +86-137 7209 8751

Fax: 029-87092164 Email: panyu1980@126.com,chuanyingpan@nwsuaf.edu.cn.

Education

B.S Northwest A&F University, Animal Science, 1999-2003

M.S Northwest A&F University, Animal Breeding, Genetics and Reproduction, 2003-2006

(Biology Engineering Research Institute of Military Medical Science Academy, Beijing, a visiting Master Degree candidate,molecular genetics, 2004-2006)

Ph.D Northwest A&F University, Genetics, 2006-2010

(Wake Forest Institute for Regenerative Medicine, Wake Forest University, USA, a visiting Ph.D Degree candidate, cell reprogramming, Sep. 2007- Sep. 2009)

Post-Doc. Northwest A&F University, Animal Breeding, Genetics and Reproduction, Mar. 2014 - Sep. 2017

Research Interests

The impacts of internal and external factors on animal reproduction and their regulatory mechanisms constitute the primary focus, encompassing: (1) Livestock reproductive cell engineering (e.g., sperm-related investigations; regulatory mechanisms of testicular interstitial cells; androgen-mediated reproductive regulation); (2) Studies on reproductive toxicity in animals; (3) Bioinformatics exploration of large-scale biological data related to animal reproduction.

Teaching

Undergraduate Courses (2010-date): (a) Cellular Biology; (b) Cellular Biology(English); (c)Cell Biology Experiments(d)Animal Genetics (e)Molecular Biology Experiment.

Graduate Courses (2013-date): (a) Advanced Cellular Biology; (b) Cell Biology Experiments; (c)Animal Cell Culture Techniques; (d) Cytogenetics.

Student Development

Appointed as a master's degree supervisor in 2012 and as a doctoral degree supervisor in 2017. Independently guided 15 master's students, among which 80% pursued doctoral degrees, with research conducted at institutions including the University of Wisconsin-Madison, Max Planck Institute in Germany, Tsinghua University, Shanghai Jiao Tong University, University of Science and Technology of China, Sun Yat-sen University, Northwest A&F University, Nanjing Medical University, among others.

Supervising 5 doctoral students and 11 master's students (including 7 accepted via recommendation). Five students received "National Graduate Scholarship", and one student received the Outstanding Master's Thesis Award at the university level.

Guided 15 university-level scientific and creative projects (including 5 at the national level), winning 16 national-level awards, 4 provincial-level awards, and over 10 university-level awards.

Additionally, supervised 37 undergraduate theses, with 2 being recognized as "Top 100 Outstanding Undergraduate Theses" by the university, and 6 receiving the university-level Outstanding Undergraduate Thesis Award and 6 the college-level award.

Funding Projects

Led over 20 research projects at the national, provincial, and university levels, as well as industry-sponsored projects. These include: (1) Three national-level projects, encompassing one National Natural Science Foundation project (General Program), one National Natural Science Foundation project (Youth Program), and one sub-project of National Key Research and Development Program; (2) Four provincial-level projects; (3) two key open projects of provincial-level key laboratories; (4) Led over ten university-level projects; (5) Additionally, led over ten projects in collaboration with institutions such as the Chinese Academy of Agricultural Sciences, Chinese Academy of Sciences, Academy of Military Science, and various enterprises; also participated in two National Natural Science Foundation projects (regional category). Moreover, as a core member, participated in projects including those funded by the National Institutes of Health (NIH), National Natural Science Foundation, National "973" sub-projects, and National "863" sub-projects.

Published over 80 papers as first author or corresponding author in SCI journals or top-tier Chinese journals, including more than 72 SCI papers across 26 different SCI journals. Among them, there are 10 papers with Impact Factor (IF) greater than 5.0, 9 highly cited or hotspot papers according to ESI, 10 papers in top journals recognized by the Chinese Academy of Sciences (CAS), and over 30 papers in CAS Zone 1 or Zone 2 journals.

Received a provincial-level Science and Technology Progress Award, acquired 2 provincial-level science and technology achievements (internationally leading), obtained authorization for 5 national software copyrights, and secured authorization for over 10 national invention patents (5 as the first author).

Partial Publications

1)Zhou Q, Cao CN, Zhang HH, Liang YL, Zhang XY, Kang YX, Fang WW, Lan XY, Li R, Pan CY * . 2023. Convergent changes in melanocortin receptor 1 gene are associated with black-headed coat color in sheep. J Anim Sci, 101:skad084. doi: 10.1093/jas/skad084.

2)Tang Q, Zhang Y, Yue L, Ren H, Pan C*. Ssc-MiR-21-5p and Ssc-MiR-615 Regulates the Proliferation and Apoptosis of Leydig Cells by Targeting SOX5. Cells, 2022, 11(14):2253.

3)Wang Qian, Bi Yi, Wang Zhiying, Zhu Haijing, Liu Mei, Wu Xianfeng, Pan Chuanying*. Goat SNX29: mRNA expression, InDel and CNV detection, and their associations with litter size. Front Vet Sci. 2022 Aug 10;9:981315. doi: 10.3389/fvets.2022.981315.

4)Kang Y, Zhu Q, Meng F, Xu H, Guo Z, Pan C*. Rapid detection of InDel within the KDM3B gene in five sheep breeds using the mathematical expectation (ME) method. Gene. 2022 Aug 5;834:146598. Epub 2022 May 20.doi: 10.1016/j.gene.2022.146598.

5)Wang Ke, Hu Huina , Cui Wenbo, Zhang Xuelian, Tang Qi , Liu Nuan , Lan Xianyong, Pan Chuanying*. Palliative effects of metformin on testicular damage induced by triptolide in male rats. Ecotoxicology and Environmental Safety, 2021 Oct 1; 222:112536. doi: 10.1016/j.ecoenv.2021.112536.

6)Bi Y#, Zhang SL#, Li J , He LB, Kang YX, Chen H, Lan XY*, Pan CY*. The mRNA expression profile of the goat prion protein testis-specific (PRNT) gene and its associations with litter size. Theriogenology 165 (2021) 69-75.

7)Bi Y, Feng WJ, Kang YX, Wang K, Yang YT, Qu L, Chen H, Lan XY*, Pan CY*. Detection of mRNA expression and copy number variations (CNVs) within the goat FecB gene associated with litter size. Frontiers in Veterinary Science. 2021; 8: 758705. doi: 10.3389/fvets.2021.758705.

8)Qi Tang, Yanghai Zhang, Yuta Yang, Huina Hu, Xianyong Lan, Chuanying Pan*. The KMT2A gene: mRNA differential expression in ovary and a novel 13-nt nucleotidesequence variant associated with litter size in cashmere goat. Domestic Animal Endocrinology，2021, 74:106538. DOI: http://doi.org/10.1016/j.domaniend.2020.106538.

9)Ke Wang, Xinfeng Liu, Tang Qi, Yiqing Hui, Hailong Yan, Lei Qu, Xianyong Lan*, Chuanying Pan*. Whole-genome sequencing to identify candidate genes for litter size and to uncover the variant function in goats (Capra hircus). Genomics, 2021,113:142-150. DOI: 10.1016/j.ygeno.2020.11.024

10)Zhang XL, Cui WB, Wang K, Chen R, Chen MY, Lan KS, Wei YP, Pan CY*, Lan XY. Chlorpyrifos inhibits sperm maturation and induces a decrease in mouse male fertility. Environmental Research. 2020,188:109785. doi: 10.1016/j.envres.2020.109785.

11)Wang K, Kang Z, Jiang E, Yan H, Zhu H, Liu J, Qu L, Lan X, Pan C*. Genetic effects of DSCAML1 identified in genome-wide association study revealing strong associations withlitter size and semen quality in goat (Capra hircus). Theriogenology. 2020, 146, 20-25.

12)Yang W, Zhao F, Chen M, Li Y, Lan X, Yang R*, Pan C*. Identification and characterization of male reproduction-related genes in pig (Sus scrofa) using transcriptome analysis. BMC Genomics. 2020; 21(1):381. doi: 10.1186/s12864-020-06790-w.

13)Cui Y, Chen R, Ma L, Yang W, Chen M, Zhang Y, Yu S, Dong W, Zeng W, Lan X, Pan C*. miR-205 Expression Elevated With EDS Treatment and Induced Leydig Cell Apoptosis by Targeting RAP2B via the PI3K/AKT Signaling Pathway. Frontiers in Cell and Developmental Biology. 2020; 8:448. doi: 10.3389/fcell.2020.00448.

14)Bi Y#, Li J#, Wang XY, He LB, Lan KS, Qu L, Lan XY*, Song XY, Pan CY*. Two novel rare missense SNPs (P27R and A85G) in GDF9 gene were strongly linked and associated with litter size in goats. Frontiers in Veterinary Science. 2020; doi: 10.3389/fvets.2020.00406.

15)Yi Bi, Bo Feng, Zhen Wang, Haijing Zhu, Lei Qu, Xianyong Lan, Chuanying Pan*, and Xiaoyue Song*. Myostatin (MSTN) Gene Indel Variation and Its Associations with Body Traits in Shaanbei White Cashmere Goat. Animals, 2020, 10, 168; doi:10.3390/ani10010168.

16)Zhang X#, Zhang S#, Tang Q, Jiang E, Wang K, Lan X*, Pan C*. Goat sperm associated antigen 17 protein gene (SPAG17): Small and large fragment genetic variation detection, association analysis, and mRNA expression in gonads. Genomics. 2020, 112(6): 5115-5121.. doi: 10.1016/j.ygeno.2020.09.029.

17)Zhen Wang, Xuelian Zhang, Enhui Jiang, Hailong Yan, Haijing Zhu, Hong Chen, Jinwang Liu, Lei Qu, Chuanying Pan*, Xianyong Lan*. InDels within Caprine IGF2BP1 intron 2 and the3’-untranslated regions are associated with goat growth traits. Animal Genetics, 2020, 51(1):117-121.

18)Li HX#, Xu HW#, Akhatayeva Z, Liu HF, Lin CJ, Han XF, Lu XF, Lan XY, Zhang QF*, Pan CY*. Novel indel variations of the sheep FecB gene and their effects on litter size. Gene. 2020;767:145176. doi: 10.1016/j.gene.2020.145176.

19)Chen M#, Wang J#, Liu N, Cui W, Dong W, Xing B*, Pan C*. Pig SOX9: Expression profiles of Sertoli cell (SCs) and a functional 18 bp indel affecting testis weight. Theriogenology. 2019, 138: 94-101. doi: 10.1016/j.theriogenology.2019.07.008.

20)Mingyue Chen,HailongYan,Ke Wang,Yang Cui,Rui Chen,Jinwang Liu, Haijing Zhu, Lei Qu, Chuanying Pan*. Goat SPEF2: Expression profile, indel variants identification and association analysis with litter size.Theriogenology,139:147-155.

21)Yanghai Zhang, Wenbo Cui, Han Yang, Min Wang, Hailong Yan, Haijing Zhu,Jinwang Liu, Lei Qu, Xianyong Lan*, Chuanying Pan* A novel missense mutation (L280V) within POU1F1 gene strongly affects litter size and growth traits in goat .Theriogenology, 135 (2019) 198-203.

22)Cui Y, Chen R, Lv X, Pan C*. Detection of coding sequence, mRNA expression and three insertions/deletions (indels) ofKDM6Agene in male pig. Theriogenology. 2019,133:10-21. doi: 10.1016/j.theriogenology.2019.04.023.

23)Mingyue Chen,Wenjing Yang, Nuan Liu, Xuelian Zhang, Wuzi Dong, Xianyong Lan, Chuanying Pan*. Pig Hsd17b3: Alternative Splice Variants Expression, Insertion/Deletion (indel) in Promoter Region and Their Associations with Male Reproductive Traits. Journal of Steroid Biochemistry and Molecular Biology, 2019, 195: 105483.

24)Yanghai Zhang, KeWang, Jinwang Liu, Haijing Zhu, Lei Qu, Hong Chen, Xianyong Lan, Chuanying Pan* and Xiaoyue Song*. An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats. Animals, 2019, 9, 1114; doi:10.3390/ani9121114.

25)Yang WJ#, Yan HL#, Wang K, Cui Y, Zhou T, Xu H, Zhu HJ, Liu JW, Lan XY, Qu L, Pan CY**, Zhang EP**. Goat PDGFRB: unique mRNA expression profile in gonad and significant association between genetic variation and litter size. Royal Society Open Science. 2019,6: 180805

26)Tang Qi, Zhang Xuelian, Wang Xinyu, Wang Ke, Yan Hailong, Lan Xianyong, Qu Lei, Zhu Haijing, Pan Chuanying*. Detection of two insertion/deletions (indels) within the ADAMTS9 gene and their associations with growth traits in goat. Small Runimant Research, 2019, 180:9-14.

27)Chen R, Cui Y, Zhang X L, Zhang Y H, Chen M Y, Zhou T, Lan X Y, Dong W Z, Pan C Y*. Chlorpyrifos Induction of Testicular-Cell Apoptosis through Generation of Reactive Oxygen Species and Phosphorylation of AMPK. J Agric Food Chem, 2018, 66: 12455-12470.

28)Cui Y#, Yan H#, Wang K, Xu H, Zhang X, Zhu H, Liu J, Qu L, Lan X*, Pan C*. (2018) Insertion/Deletion within the KDM6A gene is significantly associated with litter size in goat. Front. Genet. 9:91. doi: 10.3389/fgene.2018.00091.

29)Yanghai Zhang, Yang Cui, Xuelian Zhang, Yimin Wang, Jiayang Gao, Ting Yu, Xiaoyan Lv, Chuanying Pan*. Pig StAR: mRNA expression and alternative splicing in testis and Leydig cells, and association analyses with testicular morphology traits. Theriogenology, 2018, 118: 46-56.

30)Cui Y, Zhang Y, Wei Z, Gao J, Yu T, Chen R, Lv X, Pan C*. Pig KDM5B: mRNA expression profiles of different tissues and testicular cells and association analyses with testicular morphology traits. Gene. 2018, 650:27-33.

31)Xuelian Zhang,Hailong Yan,Ke Wang,Tong Zhou, MingyueChen, HaijingZhu, Chuanying Pan*, Enping Zhang*. GoatCTNNB1: mRNA expression profile of alternative splicing in testis and association analysis with litter size. Gene,2018, 679: 297-304.

32)Shuai Yu, Pengfei Zhang, Wuzi Dong, Wenxian Zeng, Chuanying Pan*. Identification of stem leydig cells derived from pig testicular interstitium. Stem Cells International, 2017, 2017:2740272.

33)Chen R, Du J, Ma L, Wang LQ, Xie SS, Yang CM, Lan XY, Pan CY*, Dong WZ*. Comparative microRNAome analysis of the testis and ovary of the Chinese giant salamander. Reproduction. 2017,154: 169-179.

34)Fa Ren, Shuai Yu, Rui Chen, Xiaoyan Lv, Chuanying Pan*. Identiﬁcation of a novel 12-bp insertion/deletion (indel) of iPS-related Oct4 gene and its association with reproductive traits in male piglets. Animal Reproduction Science. 2017, 178: 55-60.

35)Pan Chuanying#*, Yu Shuai#, ZHANG Peng-fei, WANG Bo, ZHU Zhen-dong, LIU Ying-ying, ZENG Wen-xian*. Effect of sucrose on cryopreservation of pig spermatogonial stem cells. Journal of Integrative Agriculture, 2017, 16(5):1120-1129.

36)ZHOU Xin-yu#, LIU Liang-liang#, JIA Wen-chao, PAN Chuan-ying*. Methylation profile of bovine Oct4 gene coding region in relation to three germ layers. Journal of Integrative Agriculture, 2016, 15(3): 618- 628.

37)CY Pan#*, WC Jia#, BS Lu, CE. Bishop*. Expression of TAT recombinant Oct4, Sox2, Lin28, and Nanog proteins from baculovirus-infected Sf9 insect cells. Gene, 2015 , 556 (2): 245-248.

38)CY Pan, CY Wu, WC Jia, Y Xu, SR Hu, CZ Lei, XY Lan*, H Chen. A critical functional missense mutation (H173R) in the bovine PROP1 gene significantly affects growth traits in cattle. Gene, 2013, 531(1): 398-402.

39)CY Pan, XY Lan, H Chen*, C.E Bishop*. An economical single-sided antibody incubation method for western blotting. Journal of Virological Methods, 2010,169:409-411.

40)CY Pan, A Hicks, H Chen*, C.E Bishop*. SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells. Journal of Genetics and Genomics. 2010, 37:241-248.

Book Chapter

1）Chuanying Pan, Xianyong Lan, Haijing Zhu，etal.2022.Cell Biology Principlesand Experimentaltecjniques.Northwest A&F UniversityPublishers.

2）Wenxian Zeng*, Chuanying Pan* and Wan-Sheng Liu. 2016. Chapter Two: Regulatory Role of Noncoding RNAs during Spermatogenesis (3 chapters in total). In: Spermatogenesis: Molecular Mechanisms, Regulation and Biological Perspectives, Edited by Gladys Robinson. Nova Science Publishers. pp. 31-70. (*These authors contributed equally to this work) (https://www.novapublishers.com)

3）Hong Chen,Chuanying Pan,etal. Chapter 17 of the third edition of "Genetic Engineering" covers Genetic Engineering Regulations and Patents.China Agriculture Press.

4）Hong Chen,Chuanying Pan,etal. Chapter Thirteenofthe"Cell Genetics"Characteristics and Research Applications of Stem Cells. Science Press.