English

Xianyong Lan

Ph.D. Xianyong Lan, Professor(Revised on August 30, 2023)

Xianyong Lan, Ph.D., Professor

College of Animal Science and Technology,

Northwest A&F University (NWAFU),

No. 22 Xinong Road,Yangling, Shaanxi Province, 712100, China

Telephone: +86-137 7207 1502

Fax: 029-87092164 Email: lan342@126.com;lanxianyong79@nwsuaf.edu.cn.

Education

B.S. degree: NorthwestMinzu University, Animal Science, 2001

M.S. degree:Northwest A&F University(NWAFU), Animal Breeding, Genetics and Reproduction, 2004

Ph.D. degree: Northwest A&F University(NWAFU), Genetics, 2007

(Meanwhile, Visiting Doctor-candidate graduate for one year in Beijing Ditan Hospital, 2005-2006)

Visiting scholar: Wake Forest Institute for Regenerative Medicine (WFIRM), Wake Forest University School of Medicine, Wake Forest University,Jul.2008 -Oct. 2008

Post-Doc:Department of Animal Science, University of Wisconsin-Madison, Nov.2011-Nov. 2012

Professional background

Sep. 2007- Dec.2009

An advanced Lecturer in Animal Breeding, Genetics& Reproduction, College of Animal Science and Technology, Northwest A&F University

Dec.2009- Dec. 2015

Associate Professor in Animal Breeding, Genetics& Reproduction,College of Animal Science and Technology, Northwest A&F University

Jan. 2016- date

Professor in Animal Breeding, Genetics& Reproduction, College of Animal Science and Technology, Northwest A&F University

Research Interests

My research interests focus on animal genetics and epigenetics. In details, research interests of genetics include genetic variations and function of DNA in livestock (especially in beef cattle, dairy cow, sheep and goat), analysis of genetic diversity and evolution. Research interests of epigenetics include detectionand functional evaluation of non-coding RNA (ncRNA, including miRNA, lncRNA and CircRNA), alternative splicing and DNA methylation in livestock. A primary focus is the functional study of SNP/Indel/CNV, alternative splicing, miRNA, lncRNA and CircRNA and DNA methylation in ruminant animals (especially in cattle, sheep and goat).

Teaching

Undergraduate Courses (2007-date): (a) Animal Genetics (Awarded “ National Classic Course” by Ministry of Education of China in 2009; awarded “National Classic Open-Course in Website” by Ministry of Education of China in 2013);(b) Animal Gene Engineering.(c) Innovation Training for Undergraduate (Shanxi Provincial certificate coursed)

Graduate Course (2009-date): (a) Molecular Genetics; (b)Cytogenetics.

Service

Reviewers for the following journals: Journal of Agricultural and Food Chemistry, Meat Science, Molecular Therapy - Nucleic Acids, JournalofPinealResearch, iScience,BMC genomics, Genomcis, Animal, Journalof Animal Science, Journal of Integrative Agriculture, Journal of Cellular Physiology, Theriogenology,Animal Genetics, Scientific Reports, PLoS One, DNA and cell biology, Gene, Small Ruminant Research, BMC genetics, Genetica, Genome, Biochemical Genetics, Food Science & Nutrition, Molecular Biology Reports, Achieves of Animal Breeding (Archiv Tierzucht), Journal of Genetics, Frontiers in cell and developmental biology, Frontiers in Veterinary Science, Frontiers in oncology, Frontiers in genetics, Frontiers in Endocrinology, Frontiers in Bioengineering and Biochemistry, Animal Biotechnology,Journal of Applied Animal Research,Anatomical Record,JournalofNorthwestA&F University (NaturalScienceEdition).

Awards

Awarded “Young New Star on Science & Technology of Shaanxi Province” by the Government Office of Shaanxi Province, China. Sep.2011.

Awarded “Young topnotch Researcher of Northwest A&F University”by the Official committee of Northwest A&F University. Sep. 2009.

Awarded “Excellent Academic Fellow of Northwest A&F University” by the Official committee of Northwest A&F University. Dec. 2008.

Awarded “President Specific Foundation about Excellent Academyfor NWSUAF Doctoral Graduation” by the President Office of Northwest A&F University. Jul. 2007.

Funds (Host)

National Natural Science Foundation of China (NSFC) (general project): (No.32372852), 2024.01-2027.12, funded by the committee of NSFC (China government);

"14th Five-Year Plan" national key research and development sub-project (the mainstay of the project)(No.2022YFF1000103-01), 2022.12-2027.11, funded by the committee of Sci-Tech (China government)

National Natural Science Foundation of China (NSFC) (general project): (No.31872331), 2019.01-2022.12, funded by the committee of NSFC (China government);

National Natural Science Foundation of China (NSFC) (general project): (No.31772400), 2017.01-2020.12, funded by the committee of NSFC (China government);

National Natural and Science Foundation of China (NSFC) (general project)(No.31172184), 2012.1-2015.12, funded by the committee of NSFC (China government);

Natural Science Foundation of Shaanxi Province (general project): 2017.01-2018.12, funded by the government of Shaanxi Province.

Natural Science Foundation of Shaanxi Province of China (No.2011JQ3009),2011.1-2013.12, funded by the government of Shaanxi Province.

Young New Star Project on Science &Technology of Shaanxi Province (No.2011kjxx64), 2011.10-2014.10, funded by the government of Shaanxi Province.

Special Fund for Basic Scientific Research and operation expenses in Sci-Tech Innovation of NWAFU (QN2011102), 2012.1-2013.12, funded by the official committee of Northwest A&F University.

Young Topnotch Researcher Support Project of Northwest A&F University(No.QNGG-2009-007), 2009.10-2012.12, funded by the official committee of Northwest A&F University.

Publications (selectedfrom peer-reviewed journals)

Published more than 150 SCI papers in more than 40 international journals as the first author or corresponding author (including co-first author or co-corresponding author), and was selected into the awardlist[LK1]- ‘Top 100 global agricultural interdisciplinary high-yield authors and high-quality papers’ in 2021.

Representative Academic Papers are listed below (#Co-first author; *Corresponding author):

[1]Zuo Wang, Yi Bi, Kemin Li, Zan Song, Chuanying Pan, Shengxiang Zhang, Xianyong Lan*, Nicholas S. Foulkes*, Haiyu Zhao*. Nickel oxide nanoparticles induce developmental neurotoxicity in zebrafish by triggering both apoptosis and ferroptosis. Environmental Science: Nano, 2023,10, 640-655.

[2]Dwi Wijayanti, Yunyun Luo, Yangyang Bai, Chuanying Pan, , Lei Qu, Zhengang Guo, Xianyong Lan*. New insight into copy number variations of goat SMAD2 gene andtheir associations with litter size and semen quality. Theriogenology 206 (2023) 114e122

[3]Huang Y#, Zhang K#, Li Y, Zhang S, Akhatayeva Z, Jiang F, Song E*, Lan X*.Bovine HOXA11 Gene Identified from RNA-Seq: mRNA Profile Analysis and Genetic Variation Detection Using ME Method and Their Associations with Carcass Traits.Cells. 2023 Feb 8;12(4):539. doi: 10.3390/cells12040539.

[4]Luo Yunyun, Akhatayeva Z, Mao C, Jiang F, Guo Z, Xu H*, Lan X*.The ovine HIAT1 gene: mRNA expression, InDel mutations, and growth trait associations.Front Vet Sci. 2023 Apr 17;10:1134903. doi: 10.3389/fvets.2023.1134903. eCollection 2023.

[5]Zhang, Sihuan, Jiang, Enhui, Kang, zihong, Bi, Yi, Liu, Hongfei, Xu, Han, Wang, Zhen, Lei, Chuzhao, Chen, Hong, Lan, Xianyong*. CircRNA profiling reveals an abundant circBDP1 that regulates bovine fat development by sponging miR-181b/miR-204 targeting Sirt1/TRARG1. Journal of Agricultural and Food Chemistry, 2022 publishedonline,doi:https://doi.org/10.1021/acs.jafc.2c05939‬‬‬‬‬‬‬

[6]Wu J, Zhang S, Yue B, Zhang S, Jiang E, Chen H, Lan X*. CircRNA profiling reveals CircPPARγ modulates adipogenic differentiation via sponging miR-92a-3p. Journal of Agricultural and Food Chemistry. 2022, 70(22): 6698-6708. (Supplementary Journal Cover)

[7]Yang Q, Han L, Li J, Xu H, Liu X, Wang X, Pan C, Lei C, Chen H, Lan X*. Activation of Nrf2 by phloretin attenuates palmitic acid-induced endothelial cell oxidative stress via AMPK-dependent signaling. Journal of Agricultural and Food Chemistry,2019, 67(1): 120-131.

[8]Han L, Li J, Li J, Pan C, Xiao Y, Lan X*, Wang M*. Activation of AMPK/Sirt3 pathway by phloretin reduces mitochondrial ROS in vascular endothelium by increasing the activity of MnSOD via deacetylation. Food & Function. 2020,11(4):3073-3083.

[9]Liu X#, Li Z#, Yan Y, Li Y, Wu H, Pei J, Yan P, Yang R*, Guo X*, Lan X*. Selection and introgression facilitated the adaptation of Chinese native endangered cattle in extreme environments. Evolutionary Applications. 2021, 14(3): 860-873.

[10]Zhang B#, Chang L#, Lan X#, Asif N, Guan F, Fu D, Li B, Yan C, Zhang H, Zhang X, Huang Y, Chen H, Yu J*, Li S*. Genome-wide definition of selective sweeps reveals molecular evidence of trait-driven domestication among elite goat (Capra species) breeds for the production of dairy, cashmere, and meat. Gigascience. 2018, 7(12): giy105.

[11]Kang Z, Zhang S, Jiang E, Wang X, Wang Z, Chen H, Lan X*. circFLT1 and lncCCPG1 sponges miR-93 to regulate the proliferation and differentiation of adipocytes by promoting lncSLC30A9 expression. Mol Ther -Nucleic Acids. 2020, 22: 484-499. (IF="10.183)

[12]Zhang M, Li B, Wang J, Zhang S, Li H, Ma L, Guo W, Lei C, Chen H, Lan X*. lnc9141-a and -b play a different role in bovine myoblast proliferation, apoptosis, and differentiation. Mol Ther -Nucleic Acids. 2019, 18: 554-566. (IF="10.18)

[13]Liu H, Akhatayeva Z, Pan C, Liao M*, Lan X*. Comprehensive comparison of two types of algorithm for circRNA detection from short-read RNA-Seq. Bioinformatics.2022, btac302. doi:10.1093/bioinformatics/btac302

[14]Zhang S, Kang Z, Cai H, Jiang E, Pan C, Dang R, Lei C, Chen H, Lan X*. Identification of novel alternative splicing of bovine lncRNA lncFAM200B and its effects on preadipocyte proliferation. Journal of cellular physiology. 2021, 236(1): 601-611.

[15]Jin Y, Wang J, Zhang M, Zhang S, Lei C, Chen H, Guo W, Lan X*. Role of bta-miR-204 in the regulation of adipocyte proliferation, differentiation, and apoptosis. J Cell Physiol. 2019, 234(7): 11037-11046.

[16]Li J, Yang Q, Han L, Pan C, Lei C, Chen H, Lan X*. C2C12 mouse myoblasts damage induced by oxidative stress is alleviated by the antioxidant capacity of the active substance phloretin. Front Cell Dev Biol. 2020, 8: 541260.

[17]Akhatayeva Z#, Cao C#, Huang Y, Zhou Q, Zhang Q, Guo Z, Tan S, Yue X, Xu H, Li R, Pan C*, Lan X*. Newly reported 90-bp deletion within the ovine BMPRIB gene: Does it widely distribute, link to the famous FecB (p.Q249R) mutation, and affect litter size? Theriogenology. 2022, 189: 222-229.

[18]Wijayanti D, Zhang S, Yang Y, Bai Y, Akhatayeva Z, Pan C, Zhu H, Qu L, Lan X*. Goat SMAD family member 1 (SMAD1): mRNA expression, genetic variants, and their associations with litter size. Theriogenology. 2022, 193:11-19

[19]Huang Y, Su P, Akhatayeva Z, Pan C, Zhang Q, Lan X*. Novel InDel variations of the Cry2 gene are associated with litter size in Australian White sheep. Theriogenology. 2022, 179: 155-161.

[20]Bi Y#, Zhang SL#, Li J, He LB, Kang YX, Chen H, Lan XY*, Pan CY*. The mRNA expression profile of the goat prion protein testis-specific (PRNT) gene and its associations with litter size. Theriogenology, 2021, 165: 69-75.

[21]Ke Wang, Xinfeng Liu, Tang Qi, Yiqing Hui, Hailong Yan, Lei Qu, Xianyong Lan*, Chuanying Pan*. Whole-genome sequencing to identify candidate genes for litter size and to uncover the variant function in goats (Capra hircus). Genomics, 2021, 113:142-150

[22]Li J, Zhang S, Shen C, Niu Z, Yang H, Zhang K, Liu Z, Wang YS*, Lan XY*. Indel mutation within the bovine HSD17B3 gene are significantly associated with ovary morphological tratis and mature number. Journal of Steroid Biochemistry and Molecular biology. 2021, 209: 105833.

[23]Zhang S, Xu H, Jiang E, Akhatayeva Z, Jiang F, Song E, Pan C, Chen H, Lan X*. Screening of bovine tissue-specific expressed genes and identification of genetic variation within an adipose tissue-specific lncRNA gene. Frontiers in Veterinary Science, 2022, 9: 887520.

[24]Bi Y, Feng WJ, Kang YX, Wang K, Yang YT, Qu L, Chen H, Lan XY*, Pan CY. Detection of mRNA expression and copy number variations (CNVs) within the goat FecB gene associated with litter size. Frontiers in Veterinary Science. 2021, 8:758705.

[25]Hui Y, Zhang Y, Wang K, Pan C, Chen H, Qu L, Song X*, Lan X*. Goat DNMT3B: An indel mutation detection, association analysis with litter size and mRNA expression in gonads. Theriogenology. 2020 Apr 15;147:108-115

[26]Kang Z, Jiang E, Wang K, Pan C, Chen H, Yan H, Zhu H, Liu J, Qu L, Lan X*. Goat membrane associated ring-CH-type finger 1 (MARCH1) mRNA expression and association with litter size. Theriogenology. 2019, 128: 8-16. （Highly cited paper of ESI, Paper on Research Front)

[27]Wang X, Yang Q, Wang K, Yan H, Pan C, Chen H, Liu J, Zhu H, Qu L, Lan X*. Two strongly linked single nucleotide polymorphisms (Q320P and V397I) in GDF9 gene are associated with litter size in cashmere goats. Theriogenology. 2019, 125: 115-121.（Highly cited paper of ESIESI, Paper on Research Front)

[28]Kang Z, Zhang S, He L, Zhu H, Wang Z, Yan H, Huang Y, Dang R, Lei C, Chen H, Qu L, Lan X*, Pan C. A 14-bp functional deletion within the CMTM2 gene is significantly associated with litter size in goat. Theriogenology. 2019, 139: 49-57.（Highly cited paper of ESI, Paper on Research Front)

[29]Zhang Y, Cui W, Yang H, Wang M, Yan H, Zhu H, Liu J, Qu L, Lan X*, Pan C*. A novel missense mutation (L280V) within POU1F1 gene strongly affects litter size and growth traits in goat. Theriogenology. 2019, 135: 198-203

[30]Zhang X, Yu S, Yang Q, Wang K, Zhang S, Pan C, Yan H, Dang R, Lei C, Chen H, Lan X*. Goat Boule: Isoforms identification, mRNA expression in testis and functional study and promoter methylation profiles. Theriogenology. 2018, 116: 53-63.

[31]Yang Q, Zhang S, Liu L, Cao X, Lei C, Qi X, Lin F, Qu W, Qi X, Liu J, Wang R, Chen H, Lan X*. Application of mathematical expectation (ME) strategy for detecting low frequency mutations: an example for evaluating 14-bp insertion/deletion (indel) within the bovine PRNP gene. Prion. 2016, 10(5): 409-419

[32]Lan X, Cretney EC, Kropp J, Khateeb K, Berg M, Peñagaricano F, Magness R, Radunz A*, Khatib H*. Maternal diet during pregnancy induces gene expression and DNA methylation changes in fetal tissues in sheep. Frontiers in genetics. 2013, 4: 49.

[33]Lan XY#, Zhao HY#, Li ZJ, Zhou R, Jiang XW, Han CS, Pan CY, Lei CZ, Chen H*. Exploring novel genetic variant of PITX1 gene and its effect on milk performance in dairy goats. Journal of Integrative Agriculture. 2013, 12(1): 118-126.

[34]Lan XY, Peñagaricano F, Dejung L, Weigel KA, Khatib H*. A missense mutation in the PROP1 (prophet of Pit 1) gene affects male fertility and milk production traits in the US Holstein population. Journal of Dairy Science. 2013, 96 (2): 1255-1257

[35]Lan XY#, Zhao HY#, Li ZJ, Li AM, Lei CZ, Chen H*, Pan CY. A novel 28-bp insertion-deletion polymorphism within goat PRNP gene and its association with production traits in Chinese native breeds. Genome. 2012 , 55(7): 547-52.

[36]Lan XY, Pan CY, Chen H*, Zhang CL, Li JY, Zhao M, Lei CZ, Zhang AL, Zhang L. An AluI PCR-RFLP detecting a silent allele at the goat POU1F1 locus and its association with production traits. Small Ruminant Research. 2007, 73: 8-12.

[37]Jiang Enhui, Liu Yuan, Li Jie, Ma Sijia, Li Xiangchen*, Lan Xianyong*. Effects of phloretin on oxidative stress and apoptosis of bovine adipose-derived stem cells induced by H2O2. Chinese Journal of Animal Nutrition, 2022, 34 (1): 600-611.

Lan Xianyong, Chen Hong, Zhang Yongde, Sun Weibin, Lei Chuchao. A New Method of Molecular Marker - RMAPD. Hereditas (Beijing), 2006, 28(1): 78- 84.