Jun Zhang

　　 　　

　　Name: Jun Zhang

Northwest A&F University, Yangling712100, P. R. China

Email:jzhang0701@nwafu.edu.cn

Phone:+86 17791651081

Research area:Physiological metabolism in ruminants,

Microbiology and health of gastrointestinal tract in ruminants

POSITION

07/2020 – presentLecturer, Ruminant Nutrition, College of Animal Science and Technology, Northwest A&F University

01/2021 – presentPostdoctor,Animal Husbandry, College of Animal Science and Technology, Northwest A&F University

EDUCATION

09/2015 – 06/2020Ph.D student, Ruminant Nutrition, College of Animal Science and Technology, China Agricultural University (CAU)

Project: Using multi-omics technologies to understand the changes related to physiological metabolism and gut microbiology in high concentrate limit-fed Holstein heifers.

08/2018 – 12/2019Visiting Scholar, Dairy nutrition and physiology, Department of Diary Science, University of Wisconsin-Madison (UW-M)

Project: Effect of insulin and EAAs interactions on mTORC1 pathway in bovine mammary gland epithelial cells.

09/2012 – 06/2015Master student,Ruminant Nutrition,Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS)

Project: New rumen ciliate primers targeting full-length 18S rRNA genes and its application in evaluating dietary effect on ciliate diversity in the rumen of dairy cows.

09/2008 – 06/2012Undergraduate student,Animal Science,College of Animal Husbandry and Veterinary, Shenyang Agricultural University (SAU)

PROJECTS

National Natural Science Foundation of China (award number: 32102570; host)

the fellowship of China Postdoctoral Science Foundation (award number:2021M702691; host)

Chinese Universities Scientific Fund (award number:2452020188; host)

Quality Control for Feed and Products of Livestock and Poultry Key Laboratory of Sichuan Province (award number:NH2021202207; host)

“Double First-Class” Funding for Animal Husbandry in China (award number:Z1010222001; host)

Science & Technological Project of Shaanxi Province (award number:2021NY-019; major participant)

Science & Technological Project of Tibet Autonomous Region (award number: XZ202202ZY0013N; major participant)

PUBLICATIONS

First authored and co-authored twenty-four peer reviewed full-textarticles and fifteen conference abstracts (requested for the full list of abstracts)

Zhang, J., Y. Yang, X. Lei, Y. Wang, Y. Li, Z. Yang, and J. Yao*. 2023. Active dry yeast supplementation benefits ruminal fermentation, bacterial community, blood immunoglobulins, and growth performance in young dairy goats, but not for intermittent supplementation. AnimNutr 13:289-301.

Zhang, J.#*, L. Deng#, X. Zhang, Y. Cao, M. Li, and J. Yao*. 2023. Multiple essential amino acids regulate mammary metabolism and milk protein synthesis in lactating dairy cows. Anim Feed Sci Technol 296: 115557.

Zhang, J., N. Gaowa, Y. Wang, H. Li, Z. Cao, H. Yang, X. Zhang, and S. Li*. 2022. Complementary hepatic metabolomics and proteomics reveal the adaptive mechanisms of dairy cows to the transition period. J Dairy Sci106:2071-2088.

Wang, G.#, J. Zhang#, S. Wu#, S. Qin, Y. Zheng, C. Xia, H. Geng, J. Yao*, and L. Deng*. 2022. The mechanistic target of rapamycin complex 1 pathway involved in hepatic gluconeogenesis through peroxisome-proliferator-activated receptor gamma coactivator-1alpha. AnimNutr 11:121-131.

Zhang, C.#, J. Zhang#, Z. Yu, G. Zhou*, and J. Yao*. 2022. Effects of supplementation with saccharomyces cerevisiae products on dairy calves: A meta-analysis. J Dairy Sci 105:7386-7398.

Pszczolkowski, V. L.#, J. Zhang#, K. A. Pignato, E. J. Meyer, M. M. Kurth, and S. I. Arriola Apelo*. 2020. Insulin potentiates essential amino acids effects on mechanistic target of rapamycin complex 1 signaling in MAC-T cells. J Dairy Sci 103(12):11988-12002.

Zhang, J.,H. Shi, Y. Wang, S. Li, Z. Cao, H. Yang, and Y. Wang*. 2020. Carbohydrate and amino acid metabolism and oxidative status in Holstein heifers precision-fed diets with different forage toconcentrate. Animal14:2315-2325.

Zhang, J., N. Zheng, W. Sheng, S. Zhao*, and J. Wang*. 2020.Synchrony degree of dietary energy and nitrogen release influences microbial community, fermentation, and protein synthesis in a rumen simulation system. Microbiology 8(2): 231.

Zhang, J.#, X. Xu#, Z. Cao, Y. Wang, H. Yang, A. Azarfar, and S. Li*. 2019. Effect of different tannins source on nutrient intake, digestibility, performance, nitrogen utilization, and blood parameters in dairy cows. Animals9(8):507.

Zhang, J., H. Shi, S. Li, Z. Cao, H. Yang, and Y. Wang*. 2019. Integrative hepatic metabolomics and proteomics reveal insights into the mechanism of different feed efficiency with high or low dietary forage levels in Holstein heifers. J Proteomics 194:1-13.

Zhang, J., H. Shi, Y. Wang, Z. Cao, H. Yang, and S. Li*. 2018. Effect of Limit-Fed Diets with Different Forage to Concentrate Ratios on Fecal Bacterial and Archaeal Community Composition in Holstein Heifers. Front Microbiol 9:976.

Zhang, J.#, H. Shi#, Y. Wang*, S. Li, H. Zhang, Z. Cao, and K. Yang*. 2018. Effects of limit-feeding diets with different forage-to-concentrate ratios on nutrient intake, rumination, ruminal fermentation, digestibility, blood parameters and growth in Holstein heifers. Anim Sci J 89(3):527-536.

Shi, H.#, J. Zhang#, S. Li, S. Ji, Z. Cao, H. Zhang, and Y. Wang*. 2018. Effects of a wide range of dietary forage-to-concentrate ratios on nutrient utilization and hepatic transcriptional profiles in limit-fed Holstein heifers. BMC Genomics 19(1):148.

Zhang, J.#, H. Shi#, Y. Wang*, S. Li, Z. Cao, S. Ji, Y. He, and H. Zhang. 2017. Effect of Dietary Forage to Concentrate Ratios on Dynamic Profile Changes and Interactions of Ruminal Microbiota and Metabolites in Holstein Heifers. Front Microbiol 8:2206.

Zhang, J.#, S. Zhao#, Y. Zhang, P. Sun, D. Bu*, and J. Wang*. 2015. New Primers Targeting Full-Length Ciliate 18S rRNA Genes and Evaluation of Dietary Effect on Rumen Ciliate Diversity in Dairy Cows. Curr Microbiol 71(6):650-657.

Pszczolkowski, V. L., H. Hu, J. Zhang, M. K. Connelly, A. S. Munsterman, and S. I. Arriola Apelo*. 2022. Effects of methionine, leucine, and insulin on circulating concentrations and mammary extraction of energy substrates and amino acids in lactating dairy cows. Domest. Anim. Endocrin. 81.

Sun, X., Y. Su, Y. Hao, J. Zhang, X. Yue, W. Wang, Z. Ma, K. Chu, S. Wang, Y. Wang, and S. Li*. 2022. Novel process methods for the whole cottonseed: Effect on the digestibility, productivity, fat profile, and milk gossypol levels in lactating dairy cows. Front Nutr 9:801712.

Hao, Y., S. Huang, G. Liu, J. Zhang, G. Liu, Z. Cao, Y. Wang, W. Wang, and S. Li*. 2021. Effects of different parts on the chemical composition, silage fermentation profile, in vitro and in situ digestibility of paper mulberry. Animals (Basel) 11.

Niu, J. L.,J. Zhang, L. Q. Wei, X. Ma, W. J. Zhang*, and C. X. Nie*. 2020.Cottonseed meal fermented by Candida tropicalis reduces the fat deposition in white-feather broilers through cecum bacteria-host metabolic cross-talk. Appl Microbiol Biotechnol 104, 4345-4357.

Huang, S, Ji, S, Yan, H, Hao, Y, Zhang, J, Wang, Y, Cao, Z and Li, S*. 2020.The day-to-day stability of the ruminal and fecal microbiota in lactating dairy cows. Microbiologyopen, e990.

Deng, Y. F.#, Y. J. Wang#, Y. Zou, A. Azarfar, X. L. Wei, S. K. Ji, J. Zhang, et al. 2017. Influence of dairy by-product waste milk on the microbiomes of different gastrointestinal tract components in pre-weaned dairy calves. Sci Rep 7:42689.

Niu, J. L., J. Zhang, L. Q. Wei, W. J. Zhang*, and C. X. Nie*. 2019. Effect of Fermented Cottonseed Meal on the Lipid-Related Indices and Serum Metabolic Profiles in Broiler Chickens. Animals (Basel) 9(11).

Ma, J., J. Yao, Y. Zhan, J. Zhang*. 2022. Research Progress of Mechanism of Antioxidants in Regulating Oxidative Stress States in Transition Dairy Cows. Chinese Journal of Animal Science.(published online; In Chinese)

Zhang, J., S. Zhao, J. Wang, D. Jin, and D. Bu*. 2015. Research progress of enzyme and relevant gene screened from rumen by metagenomic approach. Biotechnology Bulletin31 (5) :32-40. (In Chinese)

Zhang, J., S. Zhao, J. Wang, D. Bu*, D. Jin, and H. Du. 2015.Optimization of DGGE protocol in analyzing rumen bacterialcommunity diversity. Journal of Agricultural Biotechnology 09:19. (In Chinese)

Conference Oral presentation

Effects of limit-feeding different levels of dietary concentrate on ruminal bacteria and metabolic profiles in Holstein heifers. 2017, Beijing, 5th International Symposium on Dairy Cow Nutrition and Milk Quality Graduate Student Award Presentation.

New rumen ciliate primers targeting full-length 18S rRNA genes and evaluation of dietary effect on rumen ciliate diversity in dairy cows. 2015, Beijing, 4th International Symposium on Dairy Cow Nutrition and Milk Quality Graduate Student Award Presentation.

Evaluation of the effect of dietary roughage and protein sources on ruminal ciliate diversity. 2014, Nanjing, International Symposium on Gastrointestinal Microbial Ecology and Functionality.

RESEARCH COMMUNICATION

Reviewer for Animal Nutrition, Frontiers in Microbiology, Frontiers in Nutrition, Frontiers in Veterinary Science, Journal of Applied Microbiology,and Archives of Microbiology

TECHNIQUES

Laboratory Techniques

Multi-omics experience (Microbiome, Transcriptome, Proteomic, and Metabolomic)

In vitro dairy cow mammary gland epithelia cell culture,DNA and RNA extraction, primer design, Real-time PCR, Western Blot

Screening active enzyme clone from metagenomic clone library, expressing gene in BL21(DE3), SDS-PAGE, Ni-NTA purification of protein

In vitro batch culture, anaerobic culture, the rumen simulation systemoperation

Animal Experiments Techniques

Mammary gland and liver biopsy; sampling of rumen fluid, blooding, feces, urine and feeds; feed and feces composition measurement

Dairy cows and calves feeding and management, feed formulation

AWARDS

2020: Outstanding doctoral graduate.

2016-2019: First-Class Academic Scholarships, Outstanding Student Leaders, Anderson First-Class Scholarship, Boehringer Outstanding Scholarship, Merit Student.

2013-2015: Outstanding Student in Mid-Term Examination, First prize in graduate student award presentation of “4th International Symposium on Dairy Cow Nutrition and Milk Quality”.