Kun XuPh.D., A.P.
College of Animal Science & Technology
Northwest A&F University
No.22 Xinong Road, YangLing, Shaan'xi
Email: xukunas@nwafu.edu.cn，xukunas@qq.com，
Education:
2009-2015: Ph.D., Genetics, College of Animal Science and Technology, Northwest A&F University, China;
2005-2009: B.Sc., Bioengineering, College of Life Sciences, NorthwestA&F University, Shaanxi, China.
Academic Experience:
2018-Present: Associate Professor, College of Animal Science and Technology, Northwest A&F University, China;
2015-2018: Lecturer/Post-Doctor, College of Animal Science and Technology, Northwest A&F University, China.
Research Interests:
Animal Gene Editing and Molecular design Breeding: animal genome editing technology, animal mitochondrial DNA editing technology, animal molecular design breeding.
YeastGene Engineering and Animal Health Raising: microbial genetic engineering technology, microbial synthetic biology, yeastmicrocapsule technology, in vivotargeted delivery technology by oral yeast, in vivoprotein interference and immuno-Knock down, intestinal microbes and mucosal immunity.
Teaching:
Molecular biology, Animal biochemistry, Animal biotechnology, Modern animal breeding, Animal genetic engineering technology, Principles and Techniques of Gene Editing, Animal reproduction theory and biotechnology
Selected Publications:
1. Animal Gene Editing and Molecular design Breeding
[1]Lyu Ming, Sun Yongsen, Yan Nana, Chen Qiang, Wang Xin, Wei Zehui, Zhang Zhiying, Xu Kun*. Efficient CRISPR/Cas9-mediated gene editing in mammalian cells by the novel selectable traffic light reporters. Int J Biol Macromol. 2023 May; 243:124926.
[2] Ma Lixia, Xing Jiani, Li Qian, Zhang Zhiying*, Xu Kun*. Development of a universal antibiotic resistance screening reporter for improving efficiency of cytosine and adenine base editing. J Biol Chem. 2022 Jul; 298(7):102103.
[3] Ren Chonghua#, Xu Kun#, David Jay Sega, Zhang Zhiying*. Strategies for the enrichment and selection of genetically modified cells. Trends Biotechnol. 2019 Jan; 37(1):56-71.
[4] Li Xinyi, Bai Yichun, Cheng Xinzhen, Peter Girgis Tawfek Kalds, Sun Bing, Wu Yun, Lyu Huijiao, Xu Kun*, Zhang Zhiying*. Efficient SSA-mediated precise genome editing using CRISPR/Cas9. FEBS J. 2018 Sep; 285(18):3362-75.
[5] Shao Simin, Ren Chonghua, Liu Zhongtian, Bai Yichun, Chen Zhilong, Wei Zehui, Wang Xin, Zhang Zhiying*, Xu Kun*. Enhancing CRISPR/Cas9-mediated homology directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52. Int J Biochem Cell Biol. 2017 Nov; 92:43-52.
[6] Ren Chonghua#, Xu Kun#, Liu Zhongtian, Shen Juncen, Han Furong, Chen Zhilong, Zhang Zhiying*. Dual-reporter surrogate systems for efficient enrichment of genetically modified cells. Cell Mol Life Sci. 2015 Jul; 72(14):2763-72.
[7] Xu Kun, Ren Chonghua, Liu Zhongtian, Zhang Tao, Zhang Tingting, Li Duo, Wang Ling, Yan Qiang, Guo Lijun, Shen Juncen, Zhang Zhiying*. Efficient genome engineering in eukaryotes using Cas9 from Streptococcus thermophilus . Cell Mol Life Sci. 2015 Jan; 72(2):383-99.
2. Yeast Gene Engineering and Animal Health Raising
[1] Yue Feng, Du Lihong, Wang Ruyu, Han Baoquan, Zhang Xiaojun, Yao Zhangzhang, Zhang Wenqiang, Cai Chang, Zhang Zhiying*, Xu Kun*. In vivo Protein Interference Oral Administration of Recombinant Yeast-Mediated Partial Leptin Reduction for Obesity Control. Front Microbiol. 2022 Jun 14; 13:923656.
[2] Cui Jieyu, Li Yankun, Zhang Wenqiang, Qian Hongrun, Zhang Zhiying*, Xu Kun*. Alginic acid induces oxidative stress-mediated hormone secretion disorder, apoptosis and autophagy in mouse granulosa cells and ovaries. Toxicology. 2022 Feb 15;467:153099.
[3] Hafiz Muhammad Zakria, Han Baoquan, Yue Feng, Mu Lu, Fang Yuanyuan, Li Xiaoyu, Xu Kun*, Zhang Zhiying*. Significant body mass increase by oral administration of a cascade of shIL21-MSTN yeast-based DNA vaccine in mice. Biomed Pharmacother. 2019 Oct; 118:109147.
[4] Han Baoquan#, Xu Kun#,*, Liu Zhongtian, Ge Wei, Shao Simin, Li Pengcheng, Yan Nana, Li Xinyi, Zhang Zhiying*. Oral yeast-based DNA vaccine confers effective protection from Aeromonas hydrophila infection on Carassius auratus. Fish Shellfish Immunol. 2019 Jan; 84:948-54.
[5] Luo Zhixin, Wang Shanhe, Jiao Beilei, Yuan Dan, Dai Dongmei, Wang Lixian, Xu Kun*, Wang Xin*. Gene cloning and seamless site-directed mutagenesis using single strand annealing (SSA). Appl Microbiol Biotechnol. 2018 September 12; 102 (23):10119-26.
Preferred International Students
1. Languages needed: both fluent English and basic Chinese ( at least HSK2) are necessary.
2. Academic skills preferred: Molecular biology(plasmid construction, gene editing if possible), Microbiology(E. coli or yeast culturing ), Cell biology(animal cell culturing).
3. Have academic pursuits and are willing to study hard.
(Updated on 2023.09.01)
